Difference between revisions of "Part:BBa K3031008"

 
 
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<partinfo>BBa_K3031008 short</partinfo>
 
<partinfo>BBa_K3031008 short</partinfo>
  
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Linker sequence composing of GGGGS
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This part contains a rbs (part BBa_) sequence followed by the sequence for Signalling peptide Lp_0373 (part BBa_K3031003) which is flanked by two restriction sites (NdeI and Spel) creating a signalling peptide cassette which can be easily exchanged with another to test the efficiency of exporting of heterlogous proteins in lactobacillus spp.
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The protein to be exported or secreted gene of interest can be fused to the end of this sequence. Another signalling peptide can easily replace the SP by digestion and ligation for later experiments into SP efficiency.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:56, 14 October 2019


Signalling Peptide Lp_0373 + ORF81 + Cell Wall Anchor

This part contains a rbs (part BBa_) sequence followed by the sequence for Signalling peptide Lp_0373 (part BBa_K3031003) which is flanked by two restriction sites (NdeI and Spel) creating a signalling peptide cassette which can be easily exchanged with another to test the efficiency of exporting of heterlogous proteins in lactobacillus spp.

The protein to be exported or secreted gene of interest can be fused to the end of this sequence. Another signalling peptide can easily replace the SP by digestion and ligation for later experiments into SP efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 737
    Illegal AgeI site found at 636
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 857
    Illegal SapI site found at 826