Difference between revisions of "Part:BBa K143033:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks | + | LacI was located in the sequence of the ''B. subtilis'' shuttle vector pDR111. This version of LacI lacks an Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on ''B.subtilis''. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (<bbpart>BBa_K143015</bbpart>) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene. |
===Source=== | ===Source=== |
Revision as of 23:12, 18 September 2008
LacI (Lva-, N-terminal deletion) regulatory protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
LacI was located in the sequence of the B. subtilis shuttle vector pDR111. This version of LacI lacks an Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on B.subtilis. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (BBa_K143015) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene.
Source
The LacI gene was cloned from the B. subtilis shuttle vector pDR111 using Pfu DNA polymerase PCR.
References
<biblio>
- 1 pmid=16166525
</biblio>