Difference between revisions of "Part:BBa K143032"
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− | < | + | ===<big>EpsE Molecular Clutch Gene of ''B. subtilis''</big>=== |
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The epsE gene of the exopolysaccharide synthesis operon of ''B. subtilis'' has been suggested to function in a manor similar to a molecular clutch<cite>#1</cite>. If expressed inside a cell it will prevent flagellar movement causing the cell to no longer be able to swim effectively and instead only tumble. As such EpsE could potentially be used as a controller of ''B. subtilis'' movement. | The epsE gene of the exopolysaccharide synthesis operon of ''B. subtilis'' has been suggested to function in a manor similar to a molecular clutch<cite>#1</cite>. If expressed inside a cell it will prevent flagellar movement causing the cell to no longer be able to swim effectively and instead only tumble. As such EpsE could potentially be used as a controller of ''B. subtilis'' movement. | ||
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− | Although many bacterial flaggelar assemblies contain proteins that are similar in shape, there is no guarantee that the epsE gene will function correctly in any host cell other than ''B. subtilis'' | + | Though the EPS operon is normally repressed in ''B. subtilis'', it is beneficial for the original copy of epsE gene to be knocked out if EpsE is synthetically expressed. This can be achieved by integrating over the epsE gene with the epsE integration Biobricks (<bbpart>BBa_K143005</bbpart> and <bbpart>BBa_K143006</bbpart>) which contain in-frame stop codons. |
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+ | Although many bacterial flaggelar assemblies contain proteins that are similar in shape, '''there is no guarantee that the epsE gene will function correctly in any host cell other than ''B. subtilis''''' | ||
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+ | <br> | ||
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− | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>'''<big>Sequence and Features</big>'''</span> |
<partinfo>BBa_K143032 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143032 SequenceAndFeatures</partinfo> | ||
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+ | <br> | ||
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+ | ===References=== | ||
+ | <biblio> | ||
+ | #1 pmid=18566286 | ||
+ | </biblio> | ||
Revision as of 17:36, 18 September 2008
EpsE Molecular Clutch Gene of B. subtilis
The epsE gene of the exopolysaccharide synthesis operon of B. subtilis has been suggested to function in a manor similar to a molecular clutch#1. If expressed inside a cell it will prevent flagellar movement causing the cell to no longer be able to swim effectively and instead only tumble. As such EpsE could potentially be used as a controller of B. subtilis movement.
Though the EPS operon is normally repressed in B. subtilis, it is beneficial for the original copy of epsE gene to be knocked out if EpsE is synthetically expressed. This can be achieved by integrating over the epsE gene with the epsE integration Biobricks (BBa_K143005 and BBa_K143006) which contain in-frame stop codons.
Although many bacterial flaggelar assemblies contain proteins that are similar in shape, there is no guarantee that the epsE gene will function correctly in any host cell other than B. subtilis
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 762
Illegal AgeI site found at 512 - 1000COMPATIBLE WITH RFC[1000]
References
<biblio>
- 1 pmid=18566286
</biblio>