Difference between revisions of "Part:BBa K143002"

Revision as of 16:37, 18 September 2008

3' Integration Sequence for the AmyE locus of B. subtilis

Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a BioBrick construct and the 3' integration sequence specific for this locus (BBa_K143001) to the rear of the BioBrick construct to allow integration of the BioBrick construct into the chromosome of the Gram-positive bacterium B. subtilis.

The AmyE locus was the first locus used for integration into B. subtilis by Shimotsu and Henner[1] and is still commonly used in vectors such as pDR111[2], pDL[3] and their derivatives. Integration at the AmyE locus removes the ability of B. subtilis to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn[4]. For the iGEM 2008 competition, the Imperial Team used the 5' and 3' integration sequences for the AmyE locus to integrate genetic construct into the B. subtilis chromosome.

References

1 pmid=3019840
2 pmid=14597697
3 Bacillus Genetic Stock Center [www.bgsc.org]
4 Cutting, S M.; Vander-Horn, P B. Genetic analysis. In: Harwood C R, Cutting S M. , editors. Molecular biological methods for Bacillus. Chichester, England: John Wiley & Sons, Ltd.; 1990. pp. 27–74.

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 [[Image:AmyE_Integration.png|center|800px]]
-Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a Biobrick construct and the 3' integration sequence specific for this locus (<bbpart>BBa_K143001</bbpart>) to the rear of the Biobrick construct to allow integration of the Biobrick construct into the chromosome of the gram positive bacterium ''B. subtilis''.
+Integration sequences allow DNA to be incorporated into the chromosome of a host cell at a specific locus using leading (5') and trailing (3') DNA sequences that are the same as those at a specific locus of the chromosome. The 5' integration sequence can be added to the front of a BioBrick construct and the 3' integration sequence specific for this locus (<bbpart>BBa_K143001</bbpart>) to the rear of the BioBrick construct to allow integration of the BioBrick construct into the chromosome of the Gram-positive bacterium ''B. subtilis''.
-The AmyE locus was the first locus used for integration into ''B. subtilis'' by Shimotsu and Henner[1] and is still commonly used in vectors such as pDR111[2], pDL[3] and their derivatives. Integration at the AmyE locus removes the ability of ''B. subtilis'' to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn[4]. The 5' and 3' integration sequences for the AmyE locus were used to integrate the Imperial 2008 iGEM project primary construct into the ''B. subtilis'' chromosome
+The AmyE locus was the first locus used for integration into ''B. subtilis'' by Shimotsu and Henner[1] and is still commonly used in vectors such as pDR111[2], pDL[3] and their derivatives. Integration at the AmyE locus removes the ability of ''B. subtilis'' to break down starch, which can be assayed with iodine as described by Cutting and Vander-horn[4]. For the iGEM 2008 competition, the Imperial Team used the 5' and 3' integration sequences for the AmyE locus to integrate genetic construct into the ''B. subtilis'' chromosome.
 ===References===