Difference between revisions of "Part:BBa K143031:Design"
m (→References) |
m |
||
Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K143031 short</partinfo> | <partinfo>BBa_K143031 short</partinfo> | ||
+ | |||
+ | <br> | ||
<partinfo>BBa_K143031 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143031 SequenceAndFeatures</partinfo> | ||
Line 7: | Line 9: | ||
===Design Notes=== | ===Design Notes=== | ||
The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning | The sequence of ''B. subtilis'' integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning | ||
− | |||
Revision as of 13:48, 18 September 2008
Aad9 Spectinomycin Resistance Gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 488
Design Notes
The sequence of B. subtilis integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning
Source
Aad9 was PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase