Difference between revisions of "Part:BBa K143013:Design"

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===Design Notes===
 
===Design Notes===
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The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick standard was applied to the promoter P43 sequence.
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The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick prefix and suffix standard was applied to the promoter P43 sequence.
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===Source===
 
===Source===

Revision as of 13:20, 18 September 2008

Promoter 43 a constitutive promoter for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick prefix and suffix standard was applied to the promoter P43 sequence.

Source

The part was designed using the sequence from the B.subtilis genome and from previously published papers 1. This sequence was then synthesised by Geneart.

References

<biblio>

  1. 1 pmid=6330116

</biblio>