Difference between revisions of "Part:BBa K143012:Design"

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===Design Notes===
 
===Design Notes===
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The biobrick part was designed to include a single binding site for the ''B.subtilis major sigma factor. In addition the biobrick standard was applied to the promoter Pveg sequence.
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The biobrick part was designed to include a single binding site for the ''B. subtilis'' major sigma factor A. In addition the biobrick prefix and suffix standards were applied to the promoter Pveg sequence.
  
  
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===Source===
 
===Source===
  
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The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. The sequence supplied was compared to that of the DBTBS database<cite>#3</cite> then a section containing the binding site synthesised by Geneart.
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The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. The sequence supplied was compared to that of the DBTBS database<cite>#1</cite> then a section containing the binding site synthesised by Geneart.
  
 
===References===
 
===References===

Revision as of 12:56, 18 September 2008

Promoter veg a constitutive promoter for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick part was designed to include a single binding site for the B. subtilis major sigma factor A. In addition the biobrick prefix and suffix standards were applied to the promoter Pveg sequence.


Source

The Pveg promoter was suggested to us by Dr. Jan-Willem Veening of Newcastle University. The sequence supplied was compared to that of the DBTBS database#1 then a section containing the binding site synthesised by Geneart.

References

<biblio>

  1. 1 pmid=17962296

</biblio>