Difference between revisions of "Part:BBa K3102015"
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HLYA signal peptide found in the C-terminal signal sequence of alpha-hemolysin. Used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein may result in transport of the protein from the cytoplasm to the extracellular medium in a single step.
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HLYA is a signal peptide found in the C-terminal signal sequence of alpha-hemolysin. It is used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein may result in transport of the protein from the cytoplasm to the extracellular medium in a single step.
  
 
For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.
 
For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.

Revision as of 15:05, 14 October 2019

HLYA is a signal peptide found in the C-terminal signal sequence of alpha-hemolysin. It is used to target proteins for secretion via the Type I secretion pathway of gram-negative bacteria [Nicaud J. M. et al., 1986; Stanley P. et al., 1991]. Unlike Type II signal peptides, fusion of the HlyA signal peptide to the target protein may result in transport of the protein from the cytoplasm to the extracellular medium in a single step.

For our project, this protein is used to secrete both CAE and EG1 proteins by E.coli.