Difference between revisions of "Part:BBa J100034"
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===Usage and Biology===
 
===Usage and Biology===
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Several promoters with high expression level that are commonly used in Bacillus subtilis were screened to determine the most efficient ones for our experiments on the construction of biological parts. Three of such promoters, Groe (BBa J100034), SecA (BBa K1469002), P43 (BBa K208002), exist in the iGEM registry already.
  
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The primer sequences for individual promoters used for our synthetic biology project are as follows:
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groe-up  CGCGGATCCCCAATACTGTTTTCTCAAATGGTATGTA            BamHI
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groe-down  CGGGGTACCTGAAATAACCTCCTCAATAGTATGA            KpnI
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YxiE-up  CGCGGATCCAATTGAAGCGCGCGAAGCCA
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YxiE-down  CGGGGTACCGCTCTTCCCGCCTTTCGGACTG
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SecA-up  CGCGGATCCGGACATCGTCCGTCAGAAACGCTTT
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SecA-down  CGGGGTACCTCACACGCCTATTTTAGAGGCATGT
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Ylbp-up  CGCGGATCCGTCACAACAGTCACGTCGTGATAAA
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Ylbp-down  CGGGGTACCACATATATTGTAAACGCTTTATTTA
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P43-up      CGCGGATCCTGATAGGTGGTATGTTTTCG
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P43-down    CGGGGTACCTATAATGGTACCGCTATCACT
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 14:51, 14 October 2019

groE promoter

The default state of this promoter is off and can be induced by heat. It is 44 base pairs long and is located 72 bases upstream of the start site of groE. The groE gene is from E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]