Difference between revisions of "Part:BBa K143021"

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Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal <cite>1</cite>
 
Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal <cite>1</cite>
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<br>
 
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'''To get the higest level of translation from this RBS it must be connected to a coding region preceeded by a coding region prefix<cite>#1</cite>. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency'''.
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<br>
 
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
 
<partinfo>BBa_K143021 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143021 SequenceAndFeatures</partinfo>
  
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<br>
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===References===
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<biblio>
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#1 pmid=9669336
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#2 https://parts.igem.org/Assembly:RBS-CDS_issues
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</biblio>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 11:44, 18 September 2008

SpoVG ribosome binding site (RBS) for B. subtilis

Description: SpoVG is an endogenous ribosome binding site from B.subtilis. The sequence of the spoVG ribosome binding site is AAAGGUGGUGA which is complementary to the sequence UUUCCUCCACU from the 3' region of the 16s rRNA from B.subtilis. Previous research showed that the predicted binding energy of the 16s rRNA to the RBS is -19kcal 1


To get the higest level of translation from this RBS it must be connected to a coding region preceeded by a coding region prefix#1. A standard prefix will increase the distance between the RBS and the start codon, reducing translational efficiency.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 pmid=9669336
  2. 2 https://parts.igem.org/Assembly:RBS-CDS_issues

</biblio>