Difference between revisions of "Part:BBa K3102025"
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<partinfo>BBa_K3102025 short</partinfo> | <partinfo>BBa_K3102025 short</partinfo> | ||
| − | This T7-TEVp-TEV-hlyA-ter (secreted TEV) composite is engaged into the TEV protease synthetization and secretion. | + | This T7-RBS-TEVp-TEV-hlyA-ter (secreted TEV) composite is engaged into the TEV protease synthetization and secretion. |
The TEVp (BBa_K3102012) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease. | The TEVp (BBa_K3102012) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease. | ||
Latest revision as of 14:41, 14 October 2019
T7-RBS-TEVp-ter
This T7-RBS-TEVp-TEV-hlyA-ter (secreted TEV) composite is engaged into the TEV protease synthetization and secretion.
The TEVp (BBa_K3102012) and HlyA basic parts (BBa_K554002) are present in this composite. The HlyA sequence is added in C-terminal as a secretion signal peptide recognized by the bacterial secretion system. The TEV peptide signal (BBa_K3102041) is used as a recognition signal to remove the signal peptide, HlyA by the TEV protease.
This construct is enriched with T7 promoter, RBS and terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 541
Illegal NgoMIV site found at 847
Illegal AgeI site found at 202 - 1000COMPATIBLE WITH RFC[1000]