Difference between revisions of "Part:BBa K3112040"

Revision as of 14:40, 14 October 2019

Chromosomal stabilization element group example 0

Our chromosome stabilizing elements consist of three parts: homologous arms for transformation to corresponding positions, centromeres and tag for screening.

The insertion site of this element is about 10K in the protocentromere of Yarrowia lipolytica, without excessive damage to its peripheral heterochromatin structure.

Before fusion, the whole set of elements will not have a great impact on the growth and development of cells, it only plays its role in the fusion system. It can stabilize this chromosome with stable elements when the other side of the fusion system is dominant.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 750
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1837
Illegal PstI site found at 750
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1728
Illegal BglII site found at 1788
Illegal BamHI site found at 846
Illegal XhoI site found at 2642
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 750
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 750
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3112040 short</partinfo>
-Our chromosome stabilizing elements consist of three parts: homologous arms for transformation to corresponding positions, centromeres and labels for screening.
+Our chromosome stabilizing elements consist of three parts: homologous arms for transformation to corresponding positions, centromeres and tag for screening.
-The insertion site of this element is about 10K in the protocentromere of the disintegrative ester yeast, without excessive damage to its peripheral heterochromatin structure.
+The insertion site of this element is about 10K in the protocentromere of Yarrowia lipolytica, without excessive damage to its peripheral heterochromatin structure.
 Before fusion, the whole set of elements will not have a great impact on the growth and development of cells, it only plays its role in the fusion system. It can stabilize this chromosome with stable elements when the other side of the fusion system is dominant.