Difference between revisions of "Part:BBa K143031:Design"

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Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase
 
Aad9 was PCR cloned from the ''B. subtilis'' integration vector pDR111 using Pfu DNA polymerase
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===References===
 
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<biblio>
 
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#1 pmid=1659306
 
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#2 pmid=16997985
 
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#3 pmid=15060042
 
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#4 pmid=16714443
 
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</biblio>
 

Revision as of 11:38, 18 September 2008

Aad9 Spectinomycin Resistance Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 488


Design Notes

The sequence of B. subtilis integration vector pDR111 was searched for the spectinomycin resistance gene and the Biobrick standard applied to the gene sequence upon PCR cloning


Source

Aad9 was PCR cloned from the B. subtilis integration vector pDR111 using Pfu DNA polymerase