Difference between revisions of "Part:BBa K143012"

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Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor <cite>1</cite>. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes <cite>#2</cite>, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how <cite>#3</cite>. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
 
Pveg is a constitutive promoter that constitutively expresses the P43 protein in ''B.subtilis''. Pveg contains binding sites for the ''B.sutbilis'' major sigma factor <cite>1</cite>. Pveg in ''B.subtilis'' utilises two binding sites to cause high expression of genes <cite>#2</cite>, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how <cite>#3</cite>. The context with which we used the promoter Pveg is as a '''Polymerase Per Second''' (PoPS) generator.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'><big>'''Sequence and Features'''</big></span>
 
<partinfo>BBa_K143012 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K143012 SequenceAndFeatures</partinfo>
  
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===References===
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<biblio>
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#1 pmid=6181373
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#2 pmid=6820069
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#3 pmid=14651647
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</biblio>
  
 
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Revision as of 11:26, 18 September 2008

Promoter veg a constitutive promoter for B. subtilis

Pveg is a constitutive promoter that constitutively expresses the P43 protein in B.subtilis. Pveg contains binding sites for the B.sutbilis major sigma factor 1. Pveg in B.subtilis utilises two binding sites to cause high expression of genes #2, however our Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how #3. The context with which we used the promoter Pveg is as a Polymerase Per Second (PoPS) generator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

<biblio>

  1. 1 pmid=6181373
  2. 2 pmid=6820069
  3. 3 pmid=14651647

</biblio>