Difference between revisions of "Part:BBa K3132016"

 
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<partinfo>BBa_K3132016 short</partinfo>
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The second generation CAR design, which fused a scFv, a CD8a hinge, a CD8 transmembrane domain, a 4-1BB intracellular domain and a CD3ζ chain in tandem was used in our project  (Fig. 1). We designed two chimeric receptors that contain scFv derived from antibodies that recognize human EGFR and HER2. Cetuximab (anti-EGFR) and Trastuzumab (anti-HER2) were chosen because these antibodies have been found to be safe in patients when administered as targeted drugs. And therefore, these two receptors were named CTX.CAR and TTZ.CAR receptor, respectively. Unpaired cysteine 164 within the CD8a hinge region was replaced with a serine to increase CAR expression as reported previously.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3132016 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3132016 parameters</partinfo>
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Revision as of 13:44, 14 October 2019


Generalized extracellular molecule sensor platform

The second generation CAR design, which fused a scFv, a CD8a hinge, a CD8 transmembrane domain, a 4-1BB intracellular domain and a CD3ζ chain in tandem was used in our project (Fig. 1). We designed two chimeric receptors that contain scFv derived from antibodies that recognize human EGFR and HER2. Cetuximab (anti-EGFR) and Trastuzumab (anti-HER2) were chosen because these antibodies have been found to be safe in patients when administered as targeted drugs. And therefore, these two receptors were named CTX.CAR and TTZ.CAR receptor, respectively. Unpaired cysteine 164 within the CD8a hinge region was replaced with a serine to increase CAR expression as reported previously.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1477
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2163
    Illegal BglII site found at 2444
    Illegal BamHI site found at 70
    Illegal BamHI site found at 1770
    Illegal XhoI site found at 64
    Illegal XhoI site found at 991
    Illegal XhoI site found at 2303
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2234
    Illegal BsaI.rc site found at 2598
    Illegal SapI.rc site found at 1566
    Illegal SapI.rc site found at 1581