Difference between revisions of "Part:BBa K3102035"
 
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<partinfo>BBa_K3102035 short</partinfo>
 
   
 
   
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This T7-RBS-DLDII-LLDP-ter composite part is made of LLDP part (BBa_K3102014) and the DLDII operon. The LLDP enzyme allows the transport of L-lactate across the membrane of E.coli while the DLDII operon will converts lactate into pyruvate through the D-lactate dehydrogenase enzyme coding in the operon. This composite part is crucial for the overproduction of lactate and its transport into the medium.
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This construct is enriched with T7 promoter, RBS and terminator.
  
 
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Latest revision as of 13:23, 14 October 2019


T7-RBS-DLDII Operon-Ter

This T7-RBS-DLDII-LLDP-ter composite part is made of LLDP part (BBa_K3102014) and the DLDII operon. The LLDP enzyme allows the transport of L-lactate across the membrane of E.coli while the DLDII operon will converts lactate into pyruvate through the D-lactate dehydrogenase enzyme coding in the operon. This composite part is crucial for the overproduction of lactate and its transport into the medium.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4365
  • 1000
    COMPATIBLE WITH RFC[1000]