Difference between revisions of "Part:BBa K3102034"

 
 
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<partinfo>BBa_K3102034 short</partinfo>
 
<partinfo>BBa_K3102034 short</partinfo>
  
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This T7-RBS-PGL-ter composite part is made of the 6-phosphogluconolactonase enzyme which catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate in the pentose phosphate (pentose-P) pathway. This pathway is used to create ribose molecules for biosynthesis, to increase reducing power in the cell, and to metabolize some sugars. An overexpression of PGL favors the metabolic of E.coli for the production of riboflavin leading to an increase in riboflavin concentration.
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This construct is enriched with T7 promoter, RBS and terminator.
  
 
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Latest revision as of 13:23, 14 October 2019


T7-RBS-PGL-Ter

This T7-RBS-PGL-ter composite part is made of the 6-phosphogluconolactonase enzyme which catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate in the pentose phosphate (pentose-P) pathway. This pathway is used to create ribose molecules for biosynthesis, to increase reducing power in the cell, and to metabolize some sugars. An overexpression of PGL favors the metabolic of E.coli for the production of riboflavin leading to an increase in riboflavin concentration.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 575
  • 1000
    COMPATIBLE WITH RFC[1000]