Difference between revisions of "Part:BBa K3102028"

 
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This constructs is enriched with T7 promoter, RBS and terminator.
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This construct is enriched with T7 promoter, RBS and terminator.
  
  

Latest revision as of 13:14, 14 October 2019


T7-RBS-ACS operon-Ter

This T7-RBS-ACS operon-ter composite is composed of ACS operon part (BBa_K3102013) composed of three genes: actP (Transports acetate) acs (catalyzes the conversion of acetate into acetyl-CoA) and yfcH (epimerase)[Gimenez R et al., 2003]. This part is useful to increase the overexpression of a ACS and transport of the acetate. This composite is useful to increase the overexpression of the operon and thus, the uptake of acetate into the cell to metabolize it into other substrates.


This construct is enriched with T7 promoter, RBS and terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 837
    Illegal AgeI site found at 2428
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3702