Difference between revisions of "Part:BBa K3102028"
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− | This | + | This construct is enriched with T7 promoter, RBS and terminator. |
Latest revision as of 13:14, 14 October 2019
T7-RBS-ACS operon-Ter
This T7-RBS-ACS operon-ter composite is composed of ACS operon part (BBa_K3102013) composed of three genes: actP (Transports acetate) acs (catalyzes the conversion of acetate into acetyl-CoA) and yfcH (epimerase)[Gimenez R et al., 2003]. This part is useful to increase the overexpression of a ACS and transport of the acetate. This composite is useful to increase the overexpression of the operon and thus, the uptake of acetate into the cell to metabolize it into other substrates.
This construct is enriched with T7 promoter, RBS and terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 408
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 837
Illegal AgeI site found at 2428 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3702