Difference between revisions of "Part:BBa K3279003:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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The primary concern was BioBrick compatibility so the illegal sites in RFC[10] did not appear in the sequence. And the sequence was finally designed to be cloned into pACYC184-M plasmid with BamHI and HindIII.
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The sequence was designed to be cloned into pACYC184-M plasmid with BamHI and HindIII.
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===Source===
 
===Source===

Latest revision as of 12:58, 14 October 2019


lycopene beta-cyclase (CrtY)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 572
    Illegal PstI site found at 771
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 572
    Illegal PstI site found at 771
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 153
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 572
    Illegal PstI site found at 771
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 572
    Illegal PstI site found at 771
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 877
    Illegal SapI.rc site found at 700


Design Notes

The sequence was designed to be cloned into pACYC184-M plasmid with BamHI and HindIII.

Source

This part comes from Pantoea agglomerans's genomic sequence.

References