Difference between revisions of "Part:BBa K3110001:Design"
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===Source=== | ===Source=== | ||
| − | lldD was synthesized by Twist Biosciences and lldR was obtained from the genomic DNA of <i>Ecoli</I> DH5 α. These two fragments will be joined together by SOEing PCR (Spliced Overlap Extension PCR). | + | lldD was synthesized by Twist Biosciences and lldR was obtained from the genomic DNA of <i>Ecoli</I> DH5α. These two fragments will be joined together by SOEing PCR (Spliced Overlap Extension PCR). |
===References=== | ===References=== | ||
Latest revision as of 12:49, 14 October 2019
lldR+lldD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1929
Illegal AgeI site found at 567 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1366
Design Notes
The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.
Source
lldD was synthesized by Twist Biosciences and lldR was obtained from the genomic DNA of Ecoli DH5α. These two fragments will be joined together by SOEing PCR (Spliced Overlap Extension PCR).