Difference between revisions of "Part:BBa K3110001:Design"
(→‎Source)
Line 12: Line 12:
 
===Source===
 
===Source===
  
−
lldD was synthesised by Twist Biosciences and lldR was obtained from the genomic DNA of <Ecoli>.
+
lldD was synthesized by Twist Biosciences and lldR was obtained from the genomic DNA of <i>Ecoli</I> DH5alpha. These two fragments will be joined together by SOEing PCR (Spliced Overlap Extension PCR).
  
 
===References===
 
===References===

Revision as of 12:44, 14 October 2019


lldR+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1929
    Illegal AgeI site found at 567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1366


Design Notes

The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.


Source

lldD was synthesized by Twist Biosciences and lldR was obtained from the genomic DNA of Ecoli DH5alpha. These two fragments will be joined together by SOEing PCR (Spliced Overlap Extension PCR).

References