Difference between revisions of "Part:BBa K3110001:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
There is an EcoRI site, which we couldn't remove. Hence further improvement of this part is required.
+
The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.
−
 
+
  
  
 
===Source===
 
===Source===
  
−
The gene is of genomic orign.
+
lldD was synthesised by Twist Biosciences and lldR was obtained from the genomic DNA of <Ecoli>.
  
 
===References===
 
===References===

Revision as of 12:41, 14 October 2019


lldR+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1929
    Illegal AgeI site found at 567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1366


Design Notes

The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.


Source

lldD was synthesised by Twist Biosciences and lldR was obtained from the genomic DNA of <Ecoli>.

References