Difference between revisions of "Part:BBa K3110001"

 
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<partinfo>BBa_K3110001 short</partinfo>
 
<partinfo>BBa_K3110001 short</partinfo>
  
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This gene codes for L-Lactate dehydrogenase which cleaves L-Lactate to Pyruvate thus regulating levels of intracellular lactate. Our intention of using this part is to have a control over the detection threshold of lactate by the lldPRD regulatory region.
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This is a combination of lldR (the regulatory protein of lldPRD), which has a dual role of activator and repressor "and lldD (L-Lactate Dehydrogenase) which cleave L-Lactate to pyruvate. Our intention of using this part is to have a control over the detection threshold of L-Lactate by the lldPRD regulatory region.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:30, 14 October 2019


lldR+lldD

This is a combination of lldR (the regulatory protein of lldPRD), which has a dual role of activator and repressor "and lldD (L-Lactate Dehydrogenase) which cleave L-Lactate to pyruvate. Our intention of using this part is to have a control over the detection threshold of L-Lactate by the lldPRD regulatory region.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1929
    Illegal AgeI site found at 567
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1366