Difference between revisions of "Part:BBa K3110000:Design"

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===Design Notes===
 
===Design Notes===
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The gene was codon optimized.
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The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.  
  
  

Revision as of 12:19, 14 October 2019


lldD (L-Lactate Dehydrogenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 586


Design Notes

The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.


Source

lldD is of Genomic origin and will be joined with Promoter RBS and Terminator using SOEing (Spliced Overlap Extension) PCR.

References