Difference between revisions of "Part:BBa K3030016"

Revision as of 12:09, 14 October 2019

theophylline riboswitch with SD RBS + eGFP

This is a part that can test the function of the SD RBS. The theophylline riboswitch is initially designed by Markus Wieland and J�rg S. Hartig in 2008. This switch is theophylline responsive which means this switch will undergo self-cleavage at the presence of theophylline. After self-cleavaging, Shine–Dalgarno (SD) sequence will be exposed and ribosomes can bind with mRNA and translation will initiate. We use eGFP(BBa_E0040) as our reporter. The fluorescence intensity/OD. 600 can indicate the functionality of this switch. We place this part at the downstream of pBAD promoter. With the induce of L-arabinose, BL21(DE3) cells will transcribe mRNAs containing theophylline switch and eGFP coding sequence. At the presence of theophylline, eGFP protein will be translated and by detecting the fluorescence intensity/OD. 600, the functionality of this switch can be characterized.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 753

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3030016 short</partinfo>
-This is a part that can test the function of the SD RBS
+This is a part that can test the function of the SD RBS. The theophylline riboswitch is initially designed by Markus Wieland and J�rg S. Hartig in 2008. This switch is theophylline responsive which means this switch will undergo self-cleavage at the presence of theophylline. After self-cleavaging, Shine–Dalgarno (SD) sequence will be exposed and ribosomes can bind with mRNA and translation will initiate. We use eGFP(BBa_E0040) as our reporter. The fluorescence intensity/OD. 600 can indicate the functionality of this switch. We place this part at the downstream of pBAD promoter. With the induce of L-arabinose, BL21(DE3) cells will transcribe mRNAs containing theophylline switch and eGFP coding sequence. At the presence of theophylline, eGFP protein will be translated and by detecting the fluorescence intensity/OD. 600, the functionality of this switch can be characterized.
 <!-- Add more about the biology of this part here