Difference between revisions of "Part:BBa K3308081"

 
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N1 +MBP
 
N1 +MBP
  
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<!-- Add more about the biology of this part here
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[[File:T--Pittsburgh--nested inteins overview.png|900px|thumb|none|.|Figure 1. Nested Intein design overview]]
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 11:45, 14 October 2019


GB1-GTNPC-[NrdJ-1 N (1-4)]-[gp41-1 N]

N1 +MBP

File:T--Pittsburgh--nested inteins overview.png
Figure 1. Nested Intein design overview

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 408
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 106
    Illegal BsaI.rc site found at 1227
    Illegal SapI.rc site found at 1527