Difference between revisions of "Part:BBa K3282006"
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| − | 1. Donovan, R.S., Robinson, C.W. & Glick, B.R. Journal of Industrial Microbiology (1996) 16: 145. | + | 1. Donovan, R.S., Robinson, C.W. & Glick, B.R. Journal of Industrial Microbiology (1996) 16: 145. |
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| + | 2. de Boer, H. A., Comstock, L. J., & Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences of the United States of America, 80(1), 21–25. | ||
Revision as of 11:22, 14 October 2019
Tac Promoter
Tac is a hybrid promoter, which has the consensus -35 sequence from the trp promoter and the consensus -10 sequence from the lac UV5 promoter. It is reported to be 5-10 times stronger than the lac UV5 promoter and is induced using IPTG and other galactosides while not being subject to catabolite repression (1). The higher efficiency of this hybrid promoter with respect to either one of the parental promoters is attributed to the presence of consensus Pribnow box sequence in addition to consensus -35 sequence (2).
References
1. Donovan, R.S., Robinson, C.W. & Glick, B.R. Journal of Industrial Microbiology (1996) 16: 145.
2. de Boer, H. A., Comstock, L. J., & Vasser, M. (1983). The tac promoter: a functional hybrid derived from the trp and lac promoters. Proceedings of the National Academy of Sciences of the United States of America, 80(1), 21–25.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]