Difference between revisions of "Part:BBa K2923003"
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An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. | An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. | ||
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[[Image: Gua active et inactive.jpg|400px|thumb|center|Figure: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.] | [[Image: Gua active et inactive.jpg|400px|thumb|center|Figure: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.] | ||
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| + | This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand. | ||
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| + | [[Image: Aptazyme process.jpg|500px|thumb|center| Figure: Schematic graphic of Aptazyme switch-on catalytic activity]] | ||
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Revision as of 10:16, 14 October 2019
Guanine actif aptazyme
An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module.
Figure: A ribozyme module is in black. The Shine-Dalgarno (SD) is in blue. The guanine-sensing xpt aptamer (light grey) is connected to the ribozyme via a communication module (in green). The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4.]
This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]