Difference between revisions of "Part:BBa K3257106"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3257106 short</partinfo>
 
<partinfo>BBa_K3257106 short</partinfo>
  
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gag-Q-pol with T5 promoter
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This part is an expression composite of the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) and T5 Promoter. Under different regulation by different promoters, the expression level varies accordingly. 
  
 
<!-- Add more about the biology of this part here
 
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K3257106 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3257106 SequenceAndFeatures</partinfo>
  

Revision as of 09:43, 14 October 2019

gag-Q-pol with T5 Promoter-IPTG Inducible

This part is an expression composite of the Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT) and T5 Promoter. Under different regulation by different promoters, the expression level varies accordingly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 250
    Illegal BglII site found at 1362
    Illegal BglII site found at 2668
    Illegal BglII site found at 3900
    Illegal BamHI site found at 2685
    Illegal BamHI site found at 2991
    Illegal XhoI site found at 1016
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1901
    Illegal AgeI site found at 3472
  • 1000
    COMPATIBLE WITH RFC[1000]