Difference between revisions of "Part:BBa K2923020"
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Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. To make the interaction between BH3 and guanine aptazyme iGEM Strasbourg created an aptazyme bordered by MS2 and PP7 stem-loops. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.
 
Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. To make the interaction between BH3 and guanine aptazyme iGEM Strasbourg created an aptazyme bordered by MS2 and PP7 stem-loops. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.
  
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[[Image: tout Ms2-MS2-Aptazyme-PP7-PP7.jpg|500px|thumb|center| Figure 1: Plasmid overview of MS2-MS2-Aptazyme-PP7-PP7]
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[[Image: tout Ms2-MS2-Aptazyme-PP7-PP7.jpg|500px|thumb|center| Figure 1: Plasmid overview of MS2-MS2-Aptazyme-PP7-PP7
 
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This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.
 
This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.
  
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[[Image: Aptazyme switch-on process.jpg|700px|thumb|center| Figure 2: Schematic graphic of Guanine aptazyme switch-on based bacterial three-hybrid system]
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[[Image: Aptazyme switch-on process.jpg|500px|thumb|center| Figure 2: Schematic graphic of Guanine aptazyme switch-on based bacterial three-hybrid system
 
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Revision as of 09:26, 14 October 2019


MS2-MS2-Guanine actif aptazyme-PP7-PP7

Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. To make the interaction between BH3 and guanine aptazyme iGEM Strasbourg created an aptazyme bordered by MS2 and PP7 stem-loops. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.

Figure 1: Plasmid overview of MS2-MS2-Aptazyme-PP7-PP7


This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.

Figure 2: Schematic graphic of Guanine aptazyme switch-on based bacterial three-hybrid system


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
    Illegal PstI site found at 25
    Illegal PstI site found at 64
  • 1000
    COMPATIBLE WITH RFC[1000]