Difference between revisions of "Part:BBa K2923013"
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This composite part corresponds to the fusion protein between mutated LexA repressor and MS2 coat protein. iGEM Strasbourg 2019 uses this protein as a part of constructed LexA based bacterial three-hybrid. LexA with wild type DNA binding domain fused to the PP7 coat protein and LexA with the mutated version of DNA binding domain fused to the MS2 coat protein. Respectively, these two hybrid proteins specifically recognize the mutated LexA operator sequence, which contains a wild-type half-site (CTGT) and a mutated operator half-site (CCGT) described by Dimitrova et al 1998 study. This construct allows recognition of MS2-MS2-Aptazyme-PP7-PP7.  
 
This composite part corresponds to the fusion protein between mutated LexA repressor and MS2 coat protein. iGEM Strasbourg 2019 uses this protein as a part of constructed LexA based bacterial three-hybrid. LexA with wild type DNA binding domain fused to the PP7 coat protein and LexA with the mutated version of DNA binding domain fused to the MS2 coat protein. Respectively, these two hybrid proteins specifically recognize the mutated LexA operator sequence, which contains a wild-type half-site (CTGT) and a mutated operator half-site (CCGT) described by Dimitrova et al 1998 study. This construct allows recognition of MS2-MS2-Aptazyme-PP7-PP7.  
  
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[[Image: MS2-LexA et PP7-LexA.jpg|700px|thumb|center| Figure 1: Schematic graphic of Aptazyme-induced bacterial three-hybrid system based on LexA heterodimer repressor]
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[[Image: MS2-LexA et PP7-LexA.jpg|700px|thumb|center| Figure: Schematic graphic of complete Aptazyme-induced bacterial three-hybrid system based on LexA heterodimer repressor]
 
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Revision as of 08:58, 14 October 2019


MS2cp-LexaAmut

This composite part corresponds to the fusion protein between mutated LexA repressor and MS2 coat protein. iGEM Strasbourg 2019 uses this protein as a part of constructed LexA based bacterial three-hybrid. LexA with wild type DNA binding domain fused to the PP7 coat protein and LexA with the mutated version of DNA binding domain fused to the MS2 coat protein. Respectively, these two hybrid proteins specifically recognize the mutated LexA operator sequence, which contains a wild-type half-site (CTGT) and a mutated operator half-site (CCGT) described by Dimitrova et al 1998 study. This construct allows recognition of MS2-MS2-Aptazyme-PP7-PP7.

Figure: Schematic graphic of complete Aptazyme-induced bacterial three-hybrid system based on LexA heterodimer repressor]


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
    Illegal EcoRI site found at 282
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
    Illegal EcoRI site found at 282
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
    Illegal EcoRI site found at 282
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
    Illegal EcoRI site found at 282
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal EcoRI site found at 282
  • 1000
    COMPATIBLE WITH RFC[1000]