Difference between revisions of "Part:BBa K3018000"

 
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This part encodes a bacterial flavin-containing monooxygenase(FMO), which can catalyze the oxidation of indole. We had the codon-optimized for E. coli. You can use this part to produce indigo through indoxyl and leucoindigo in E. coli.  
 
This part encodes a bacterial flavin-containing monooxygenase(FMO), which can catalyze the oxidation of indole. We had the codon-optimized for E. coli. You can use this part to produce indigo through indoxyl and leucoindigo in E. coli.  
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Indigo is a hydrophobic blue dye; the expression of functional FMO can be estimated by the color of culture solution. Clones on LB plates are blue.
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Indigo is a hydrophobic blue dye; the expression of functional FMO can be estimated by the color of culture solution. Clones on LB plates should be blue when it works.
  
 
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Revision as of 07:24, 14 October 2019


Bacterial flavin-containing monooxydase, codon-optimized

This part encodes a bacterial flavin-containing monooxygenase(FMO), which can catalyze the oxidation of indole. We had the codon-optimized for E. coli. You can use this part to produce indigo through indoxyl and leucoindigo in E. coli. Indigo is a hydrophobic blue dye; the expression of functional FMO can be estimated by the color of culture solution. Clones on LB plates should be blue when it works.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]