Difference between revisions of "Part:BBa K3140004"
Line 17: Line 17:
  
 
[[Image:T--Sydney_Australia--PsiD_KEGG_rxn2.gif|thumb|900px|Fig. 2: Decarboxylation of 4-hydroxy-L-tryptophan to 4-hydroxytryptamine, mediated by PsiD. CO<sub>2</sub> is released as a byproduct. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R11932 KEGG]  ]]
 
[[Image:T--Sydney_Australia--PsiD_KEGG_rxn2.gif|thumb|900px|Fig. 2: Decarboxylation of 4-hydroxy-L-tryptophan to 4-hydroxytryptamine, mediated by PsiD. CO<sub>2</sub> is released as a byproduct. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R11932 KEGG]  ]]
 +
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 06:57, 14 October 2019


PsiH (codon optimised) - Cytochrome P450 monooxygenase from Psilocybe cubensis

PsiH (codon optimised) is a cytochrome P450 monooxygenase, which catalyses the conversion of tryptamine to 4-hydroxytryptamine.

Usage and Biology

PsiH (codon optimised) is an enzyme derived from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. The coding sequence has been codon optimised for expression in Escherichia coli. It accepts tryptamine as a substrate to yield 4-hydroxytryptamine, respectively. Heterologous expression of PsiH has been achieved in Escherichia coli <add strain> in a <expression system>.

Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO2 is released as a byproduct. Source: KEGG


Fig. 2: Decarboxylation of 4-hydroxy-L-tryptophan to 4-hydroxytryptamine, mediated by PsiD. CO2 is released as a byproduct. Source: KEGG


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]