Difference between revisions of "Part:BBa K3140003"
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PsiM is a native enzyme obtained from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts norbaeocystin as a substrate to yield psilocybin through N,N-dimethylation. Heterologous expression of PsiM has been achieved in ''Escherichia coli'' <add strain> in a <expression system>.
 
PsiM is a native enzyme obtained from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts norbaeocystin as a substrate to yield psilocybin through N,N-dimethylation. Heterologous expression of PsiM has been achieved in ''Escherichia coli'' <add strain> in a <expression system>.
  
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[[Image:T--Sydney_Australia--PsiD_KEGG_rxn1.gif|thumb|900px|Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO<sub>2</sub> is released as a byproduct. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R00685 KEGG]  ]]
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[[Image:T--Sydney_Australia--PsiM_KEGG_rxn.gif|thumb|900px|Fig. 1: N,N-dimethylation of norbaeocystin to psilocybin, mediated by PsiM. Intermediates not shown. Two ''S''-adenosyl-L-methionine moieties are consumed, releasing two ''S''-sdenosyl-L-homocysteine moieties as a by-product. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+ R11934 KEGG]  ]]
  
  

Revision as of 06:56, 14 October 2019


PsiM - Norbaeocystin methyltransferase from Psilocybe cubensis

PsiM is a norbaeocystin methyltransferase, which catalyses the conversion of norbaeocystin to psilocybin.

Usage and Biology

PsiM is a native enzyme obtained from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts norbaeocystin as a substrate to yield psilocybin through N,N-dimethylation. Heterologous expression of PsiM has been achieved in Escherichia coli <add strain> in a <expression system>.

Fig. 1: N,N-dimethylation of norbaeocystin to psilocybin, mediated by PsiM. Intermediates not shown. Two S-adenosyl-L-methionine moieties are consumed, releasing two S-sdenosyl-L-homocysteine moieties as a by-product. Source: R11934 KEGG


ADD: -SDS-PAGE - pCW - LC/MAS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]