Difference between revisions of "Part:BBa K3140003"
| Line 4: | Line 4: | ||
PsiM is a norbaeocystin methyltransferase, which catalyses the conversion of norbaeocystin to psilocybin. | PsiM is a norbaeocystin methyltransferase, which catalyses the conversion of norbaeocystin to psilocybin. | ||
| + | |||
| + | *NCBI: [https://www.ncbi.nlm.nih.gov/protein/ASU62238.1 ASU62238.1] | ||
| + | *UniProt: [https://www.uniprot.org/uniprot/P0DPA9 P0DPA9] | ||
| + | *EC number: [https://enzyme.expasy.org/EC/2.1.1.345 2.1.1.345] | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
PsiM is a native enzyme obtained from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts norbaeocystin as a substrate to yield psilocybin through N,N-dimethylation. Heterologous expression of PsiM has been achieved in ''Escherichia coli'' <add strain> in a <expression system>. | PsiM is a native enzyme obtained from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts norbaeocystin as a substrate to yield psilocybin through N,N-dimethylation. Heterologous expression of PsiM has been achieved in ''Escherichia coli'' <add strain> in a <expression system>. | ||
| + | |||
| + | [[Image:T--Sydney_Australia--PsiD_KEGG_rxn1.gif|thumb|900px|Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO<sub>2</sub> is released as a byproduct. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R00685 KEGG] ]] | ||
| + | |||
ADD: | ADD: | ||
Revision as of 06:51, 14 October 2019
PsiM - Norbaeocystin methyltransferase from Psilocybe cubensis
PsiM is a norbaeocystin methyltransferase, which catalyses the conversion of norbaeocystin to psilocybin.
- NCBI: ASU62238.1
- UniProt: P0DPA9
- EC number: 2.1.1.345
Usage and Biology
PsiM is a native enzyme obtained from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts norbaeocystin as a substrate to yield psilocybin through N,N-dimethylation. Heterologous expression of PsiM has been achieved in Escherichia coli <add strain> in a <expression system>.
Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO2 is released as a byproduct. Source: KEGG
ADD:
-SDS-PAGE - pCW - LC/MAS
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]