Difference between revisions of "Part:BBa K3140002"
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<partinfo>BBa_K3140002 short</partinfo>
 
<partinfo>BBa_K3140002 short</partinfo>
  
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PsiK is a 4-hydroxytryptamine kinase, which catalyses the conversion of 4-hydroxytryptamine to norbaeocystin.  
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PsiK is a 4-hydroxytryptamine kinase, which catalyses the conversion of 4-hydroxytryptamine to norbaeocystin.
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*NCBI: [https://www.ncbi.nlm.nih.gov/protein/ASU62237.1 ASU62237.1]
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*UniProt: [https://www.uniprot.org/uniprot/P0DPA8 P0DPA8]
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*EC number: [https://enzyme.expasy.org/EC/2.7.1.222 2.7.1.222]
  
 
===Usage and Biology===
 
===Usage and Biology===
  
 
PsiK is a native enzyme obtained from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both 4-hydroxytryptamine and psilocin as substrates to yield norbaeocystin and psilocybin, respectively. Heterologous expression of PsiK has been achieved in ''Escherichia coli'' <add strain> in a <expression system>.
 
PsiK is a native enzyme obtained from ''Psilocybe cubensis'', which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both 4-hydroxytryptamine and psilocin as substrates to yield norbaeocystin and psilocybin, respectively. Heterologous expression of PsiK has been achieved in ''Escherichia coli'' <add strain> in a <expression system>.
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[[Image:T--Sydney_Australia--PsiD_KEGG_rxn1.gif|thumb|900px|Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO<sub>2</sub> is released as a byproduct. Source: [https://www.genome.jp/dbget-bin/www_bget?reaction+R00685 KEGG]  ]]
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Revision as of 06:45, 14 October 2019


PsiK - 4-hydroxytryptamine kinase from Psilocybe cubensis

PsiK is a 4-hydroxytryptamine kinase, which catalyses the conversion of 4-hydroxytryptamine to norbaeocystin.

Usage and Biology

PsiK is a native enzyme obtained from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both 4-hydroxytryptamine and psilocin as substrates to yield norbaeocystin and psilocybin, respectively. Heterologous expression of PsiK has been achieved in Escherichia coli <add strain> in a <expression system>.

Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. CO2 is released as a byproduct. Source: KEGG


ADD: -SDS-PAGE - pCW - LC/MAS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 864
    Illegal SapI.rc site found at 973