Difference between revisions of "Part:BBa K2936013"

Revision as of 03:54, 14 October 2019

Formaldehyde degradation system

Formaldehyde reaction of formic acid need Glutathione-dependent Formaldehyde-activating Enzyme (GFA), Glutathione-dependent formaldehyde dehydrogenase (GS-FDH) and S-formylglut-athione hydrolase (FGH) which come from Paracoccus denitrificans (p. denitrificans) catalyst. GFA can act as a glutathione (GSH) carrier to catalyze the reaction of GSH and CH2O to form s-hydroxy-methylglutathione (HMG)[1], GS-FDH can catalyze the oxidation of HMG dehydrogenation to form s-formylglutathione, and FGH can regenerate GSH.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 904
Illegal PstI site found at 1384
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 904
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 1384
Illegal NotI site found at 1745
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 904
Illegal BglII site found at 1265
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 904
Illegal PstI site found at 1384
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 904
Illegal PstI site found at 1384
Illegal NgoMIV site found at 853
Illegal NgoMIV site found at 1049
Illegal NgoMIV site found at 1402
Illegal NgoMIV site found at 1662
Illegal NgoMIV site found at 2138
Illegal NgoMIV site found at 2249
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
-Enzymes expressed by gfa, fghA and flhA gradually degraded formaldehyde into formic acid.
+Enzymes expressed by gfa, fghA and flhA gradually degraded formaldehyde into formic acid.Moreover, it can be used on plasmids and genomes.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>