Difference between revisions of "Part:BBa K143036"

Revision as of 14:15, 17 September 2008

Xylose operon regulatory protein

Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). Many repressor proteins can be inactivated by addition of an inducer, such as xylose.

XylR if the regulatory protein for the Xylose operon in B. subtilis#1 and is responsible for ensuring the xylose metabolism proteins are not expressed in the absence of xylose . Though XylR is endogenous to B. subtilis, XylR should be over-expressed to minimise the leakage of a xylose inducible promoter. In the presence of xylose, the XylR multimer is unable to bind DNA and so transcription resumes.

It must be noted that in all B. subtilis strains that do not have the xylose operon knocked out the xylose inducer will gradually be metabolised by the host.

XylR was used in conjunction with the Xylose operon promoter (BBa_K143014) and acted as an input adaptor for a Polymerases per second (POPS) output.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K143036 short</partinfo>
-Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (reppresors). Some repressor proteins can be inactivted however by addition of an inducer, such as xylose.
+Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). Many repressor proteins can be inactivated by addition of an inducer, such as xylose.
-XylR if the regulator protein for the Xylose operon in ''B. subtilis''<cite>#1</cite> and is responsible for ensuring that in the absence of xylose the xylose metabolism proteins are not expressed. Though endogenous to ''B. subtilis'', to minimise the leakage of a xylose inducible promoter XylR should be over-expressed. In the presence of xylose, the XylR tetramer is unable to bind DNA and so transcription resumes.
+XylR if the regulatory protein for the Xylose operon in ''B. subtilis''<cite>#1</cite> and is responsible for ensuring the xylose metabolism proteins are not expressed in the absence of xylose . Though XylR is endogenous to ''B. subtilis'', XylR should be over-expressed to minimise the leakage of a xylose inducible promoter. In the presence of xylose, the XylR multimer is unable to bind DNA and so transcription resumes.
-It must be noted that in all ''B. subtilis'' strains that do not have the Xylose operon knocked out '''the xylose inducer will gradually be metabolised by the host'''
+It must be noted that in all ''B. subtilis'' strains that do not have the xylose operon knocked out '''the xylose inducer will gradually be metabolised by the host'''.
-XylR was used in conjunction with the '''Xylose operon promoter''' (<bbpart>BBa_K143014</bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output
+XylR was used in conjunction with the '''Xylose operon promoter''' (<bbpart>BBa_K143014</bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output.
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