Difference between revisions of "Part:BBa K143033:Design"

m (→‎Design Notes)
m (→‎Source)
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
The LacI gene was cloned from''B. subtilis'' shuttle vector pDR111 using Pfu DNA polymerase PCR
+
The LacI gene was cloned from the ''B. subtilis'' shuttle vector pDR111 using Pfu DNA polymerase PCR
  
 
===References===
 
===References===

Revision as of 14:10, 17 September 2008

LacI (Lva-, N-terminal deletion) regulatory protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LacI was located in the sequence of the B. subtilis shuttle vector pDR111. This version of LacI lacks a Lva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on B.subtilis. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (BBa_K143015) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene.

Source

The LacI gene was cloned from the B. subtilis shuttle vector pDR111 using Pfu DNA polymerase PCR

References

<biblio>

  1. 1 pmid=16166525

</biblio>