Difference between revisions of "Part:BBa K143033"
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<partinfo>BBa_K143033 short</partinfo> | <partinfo>BBa_K143033 short</partinfo> | ||
− | LacI is a regulatory protein responsible for the repression of many catabolite genes. Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription ( | + | LacI is a regulatory protein responsible for the repression of many catabolite genes. Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (repressors). Many repressor proteins are inactivated by addition of an inducer, such as IPTG or certain sugars. |
− | LacI | + | LacI is the regulator protein for the lactose operon in ''E.coli'' and the hyper-spank promoter of ''B. subtilis''<cite>#1</cite>(<bbpart>BBaK143015</bbpart>) and is responsible for ensuring there is no expression through these promoters in the absence of lactose (or IPTG). LacI is not endogenous to ''B. subtilis'' so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes. |
− | This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart)> and | + | This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart)> and its derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E.coli'' (in differing strains). |
LacI was used in conjunction with the '''Hyper-spank promoter''' (<bbpart>BBa_K143015</bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output | LacI was used in conjunction with the '''Hyper-spank promoter''' (<bbpart>BBa_K143015</bbpart>) and acted as an input adaptor for a '''Polymerases per second''' (POPS) output |
Revision as of 14:08, 17 September 2008
LacI (Lva-, N-terminal deletion) regulatory protein
LacI is a regulatory protein responsible for the repression of many catabolite genes. Transcription is regulated by proteins which bind operator sequences around the transcription start site. These proteins can positively affect transcription (activators) or negatively affect transcription (repressors). Many repressor proteins are inactivated by addition of an inducer, such as IPTG or certain sugars.
LacI is the regulator protein for the lactose operon in E.coli and the hyper-spank promoter of B. subtilis#1(BBaK143015) and is responsible for ensuring there is no expression through these promoters in the absence of lactose (or IPTG). LacI is not endogenous to B. subtilis so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes.
This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI (BBa_C0012) and acted as an input adaptor for a Polymerases per second (POPS) output
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]