Difference between revisions of "Part:BBa K143036:Design"

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===Design Notes===
 
===Design Notes===
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The XylR protein was identified in the genome using its Genbank entry<cite>#2</cite> and NCBI's sequence viewer and PCR primers designed from the sequence. Biobrick prefix and suffix sequences were added and the gene cloned by PCR with Pfu DNA polymerase
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The XylR protein was identified in the genome using its Genbank entry[2] and NCBI's sequence viewer and PCR primers designed from the sequence. Biobrick prefix and suffix sequences were added and the gene cloned by PCR with Pfu DNA polymerase
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===Source===
 
===Source===

Revision as of 14:00, 17 September 2008

Xylose operon regulatory protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The XylR protein was identified in the genome using its Genbank entry[2] and NCBI's sequence viewer and PCR primers designed from the sequence. Biobrick prefix and suffix sequences were added and the gene cloned by PCR with Pfu DNA polymerase

Source

The XylR protein was PCR cloned form the B. subtilis genome using Pfu DNA polymerase

References

<biblio>

  1. 1 pmid=2544559
  2. 2 http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene, Gene ID:939531

</biblio>