Difference between revisions of "Part:BBa K3076150:Design"

 
 
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__NOTOC__
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<partinfo>BBa_K3076150 short</partinfo>
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<partinfo>BBa_K3076150 SequenceAndFeatures</partinfo>
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===Source===
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The <i>CgMT</i> sequence was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906). Then, codon-optimized for <i>E. coli</i> by the ThermoFisher GeneArt platform.
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===Design Notes===
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There was a PstI site at 172 bp to 177 bp after codon optimization, so the codonCTG was changed to CTA which both codon codes for leucine.
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When synthesized into the expression vector, the start codon of this part was removed because the vector contains its own start codon.
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===References===
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[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064

Latest revision as of 23:26, 13 October 2019


Coding sequence of metallothioneins (MT) gene from Corynebacterium glutamicum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 395
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

The CgMT sequence was identified by a literature review [1] and extracted from NCBI (ACCESSION: KJ638906). Then, codon-optimized for E. coli by the ThermoFisher GeneArt platform.

Design Notes

There was a PstI site at 172 bp to 177 bp after codon optimization, so the codonCTG was changed to CTA which both codon codes for leucine. When synthesized into the expression vector, the start codon of this part was removed because the vector contains its own start codon.

References

[1] Jafarian, V., & Ghaffari, F. (2017). A unique metallothionein-engineered in Escherichia coli for biosorption of lead, zinc, and cadmium; absorption or adsorption? Microbiology, 86(1), 73–81. doi: 10.1134/s0026261717010064