Difference between revisions of "Part:BBa K3027003"

 
 
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<partinfo>BBa_K3027003 short</partinfo>
 
<partinfo>BBa_K3027003 short</partinfo>
  
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Plasmid pBAD24 with the A1 nuclease CDS inserted behind the ARA promotor
+
Plasmid pBAD24 with the A1 nuclease CDS inserted downstream of the arabinose-inducible promoter P<sub>BAD</sub>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:16, 13 October 2019


pBAD24-nuclease_A1

Plasmid pBAD24 with the A1 nuclease CDS inserted downstream of the arabinose-inducible promoter PBAD

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1311
    Illegal PstI site found at 3018
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1311
    Illegal NheI site found at 1300
    Illegal PstI site found at 3018
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1311
    Illegal BamHI site found at 1239
    Illegal BamHI site found at 1331
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1311
    Illegal PstI site found at 3018
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1311
    Illegal PstI site found at 3018
    Illegal NgoMIV site found at 4581
    Illegal NgoMIV site found at 6159
    Illegal AgeI site found at 1074
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3202
    Illegal BsaI site found at 4269
    Illegal SapI site found at 1056
    Illegal SapI.rc site found at 5788