Difference between revisions of "Part:BBa K2912004:Design"
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===Design Notes===
 
===Design Notes===
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Since there is an EcoRI restriction endonuclease site on the HWP1 gene sequence, we had to use the Gibson Assembly method to clone the HWP1 gene into the BioBrick plasmid pSB1C3.
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The R-body is a killing trait used in Paramecium
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−
 
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===Source===
 
===Source===
  
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Candida albicans
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This part is DNA sequences encoding the R body locus (reb) from Caedibacter taeniospiralis and the sequence is from gene bank.
  
 
===References===
 
===References===
 +
Heruth D P , Pond F R , Dilts J A , et al. Characterization of genetic determinants for R body synthesis and assembly in Caedibacter taeniospiralis 47 and 116.[J]. Journal of Bacteriology, 1994, 176(12):3559-3567.

Revision as of 22:26, 13 October 2019


Refractile inclusion bodies


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The R-body is a killing trait used in Paramecium

Source

This part is DNA sequences encoding the R body locus (reb) from Caedibacter taeniospiralis and the sequence is from gene bank.

References

Heruth D P , Pond F R , Dilts J A , et al. Characterization of genetic determinants for R body synthesis and assembly in Caedibacter taeniospiralis 47 and 116.[J]. Journal of Bacteriology, 1994, 176(12):3559-3567.