Difference between revisions of "Part:BBa K3147005:Design"

 
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__NOTOC__
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<partinfo>BBa_K3147005 short</partinfo>
  
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<partinfo>BBa_K3147005 SequenceAndFeatures</partinfo>
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===Design Note===
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The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence.
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The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G (BBa_ J18918) with \ indicating cleaved peptide binding.
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The Terminator used is a double terminator (BBa_0015).

Revision as of 21:18, 13 October 2019

TEV protease with TEV-cleavable maltose binding protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Note

The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence. The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G (BBa_ J18918) with \ indicating cleaved peptide binding. The Terminator used is a double terminator (BBa_0015).