Difference between revisions of "Part:BBa K3147005:Design"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K3147005 short</partinfo> | ||
+ | <partinfo>BBa_K3147005 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | |||
+ | ===Design Note=== | ||
+ | |||
+ | The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence. | ||
+ | The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G (BBa_ J18918) with \ indicating cleaved peptide binding. | ||
+ | The Terminator used is a double terminator (BBa_0015). |
Revision as of 21:18, 13 October 2019
TEV protease with TEV-cleavable maltose binding protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 379
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Note
The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence. The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G (BBa_ J18918) with \ indicating cleaved peptide binding. The Terminator used is a double terminator (BBa_0015).