Difference between revisions of "Part:BBa K3142002"
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<partinfo>BBa_K3142002 short</partinfo>
 
<partinfo>BBa_K3142002 short</partinfo>
  
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Effectively secretes proteins from lactic acid bacteria to the outside of the cell.The secretory signal peptide of the secreted protein usp45 gene of Lactococcus lactis MG1363 and the coding sequence of the first 11 amino acids of the mature peptide.
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'''what it is'''：Lac repressor (lacR) and operator sequences (O1 and O2) is part of the lactose operon derived from Lactobacillus delbrueckii. O1 and O2 may serve as operators for the binding of the lac repressor (lacR).  
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'''what it does'''：O1 and O2 may serve as operators for the binding of the lac repressor (LacR) to prevent repressing the expression of downstream genes.
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'''how to use it in other projects'''：BBa_K609001. When the external pH value declines to 5.5, rcfB promoter will be highly upregulated. Along with that, the lacR gene, which is downstream of rcfB will be highly transcribed. The large translation of LacR, the repressor of lac operon in Lactobacillus delbrueckii, will inhibit the use of lactose in the bacteria.
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<partinfo>BBa_K3142002 parameters</partinfo>
 
<partinfo>BBa_K3142002 parameters</partinfo>
 
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===生长曲线===
 
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这个图片是我们测试得到的生长曲线
 
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[[Image:WuzhetangSTR.png|right||500px]]
 

Revision as of 19:05, 13 October 2019


Acid-induced promoter and repressor lacR of lac operon

what it is：Lac repressor (lacR) and operator sequences (O1 and O2) is part of the lactose operon derived from Lactobacillus delbrueckii. O1 and O2 may serve as operators for the binding of the lac repressor (lacR).

what it does：O1 and O2 may serve as operators for the binding of the lac repressor (LacR) to prevent repressing the expression of downstream genes.

how to use it in other projects：BBa_K609001. When the external pH value declines to 5.5, rcfB promoter will be highly upregulated. Along with that, the lacR gene, which is downstream of rcfB will be highly transcribed. The large translation of LacR, the repressor of lac operon in Lactobacillus delbrueckii, will inhibit the use of lactose in the bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal NgoMIV site found at 149
    Illegal NgoMIV site found at 1052
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 989
    Illegal SapI.rc site found at 718