Difference between revisions of "Part:BBa K3019013:Design"

 
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===Source===
 
===Source===
  
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BBa_K2711000 is taken from iGEM registry. Pre-Ost1-pro-α-factor signal sequence was taken from yeast genomic DNA and 3HA tag sequence was kindly provided by professor Mart Loog research group. 3HA tag was placed to pre-pro signal C-terminal. The whole sequence was ordered from IDT.
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BBa_K2711000 sequence is taken from iGEM registry. Pre-Ost1-pro-α-factor signal sequence was taken from yeast genomic DNA and 3HA tag sequence was kindly provided by professor Mart Loog research group. 3HA tag was placed to pre-pro signal C-terminal. The whole sequence was ordered from IDT.
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===References===
 
===References===

Latest revision as of 18:00, 13 October 2019


BBa_2711000 Pre-Ost1-pro-α-factor signal 3HA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 480
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 595
    Illegal BamHI site found at 815
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 484
    Illegal NgoMIV site found at 846
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Illegal cutting sites removal


Source

BBa_K2711000 sequence is taken from iGEM registry. Pre-Ost1-pro-α-factor signal sequence was taken from yeast genomic DNA and 3HA tag sequence was kindly provided by professor Mart Loog research group. 3HA tag was placed to pre-pro signal C-terminal. The whole sequence was ordered from IDT.

References