Difference between revisions of "Part:BBa K3257113"

Revision as of 17:42, 13 October 2019

T7 Promoter-lox5171-R-PPT-RBS-ChlR_Y92X-PBS-U5-R-loxP-T7 Terminator

This part includes the gene of interest, which is ChlR_Y92X (https://parts.igem.org/Part:BBa_K3257036) in this part, and a set of necessary regulatory factor for the reverse transcription and recombination, such as the primer binding site (PBS https://parts.igem.org/Part:BBa_K3257063), the R region (https://parts.igem.org/Part:BBa_K3257061), the U5 region (https://parts.igem.org/Part:BBa_K3257062), the polypurine tract (PPT https://parts.igem.org/Part:BBa_K3257060) and lox sites (https://parts.igem.org/Part:BBa_K3257064 https://parts.igem.org/Part:BBa_K3257075). Together they function as a target of the reverse transcription and recombination process, making them vital and fundamental for our R-Evolution system.

Sequence and Features