Difference between revisions of "Part:BBa K3142000"
| Line 18: | Line 18: | ||
<partinfo>BBa_K3142000 parameters</partinfo> | <partinfo>BBa_K3142000 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | ===Results=== | ||
| + | The A11Sg-AHPM protein was carried out in the lactococcus lactis MG1363 harboring the pMG36e-rcfB-O1/O2-SPusp45-A11Sg-AHPM vector, which was grown in the GM17 broth under different pH. In the 12%SDS-PAGE, one band of size 64 kDa corresponding to the recombinant modified protein was detected at the pH=6. | ||
| + | [[file:PH01.png|500px|center]] | ||
Revision as of 17:31, 13 October 2019
Acid-induced promoter（PrcfB）
The rcfB promoter, a member of the CRP-FNR positive regulation family, was amplified from chromosomal DNA lactis subsp. lactis IL1403. It is an important part of the P170 promoter regulated by acid. When the external pH value declines to 5.5, rcfB promoter will be highly upregulated.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 9
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 9
Illegal NotI site found at 15 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 9
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 9
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 9
Illegal XbaI site found at 24 - 1000COMPATIBLE WITH RFC[1000]
Results
The A11Sg-AHPM protein was carried out in the lactococcus lactis MG1363 harboring the pMG36e-rcfB-O1/O2-SPusp45-A11Sg-AHPM vector, which was grown in the GM17 broth under different pH. In the 12%SDS-PAGE, one band of size 64 kDa corresponding to the recombinant modified protein was detected at the pH=6.
