Difference between revisions of "Part:BBa K3112002"

 
 
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<partinfo>BBa_K3112002 short</partinfo>
 
<partinfo>BBa_K3112002 short</partinfo>
  
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The first part of Cas9.We made a point mutation and codon optimization in real synthesis and transformed it into dcas9.
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dcas9 is a mutant of cas9 protein. The simultaneous mutation of RuvC1 and HNH nuclease activity regions in cas9 endonuclease resulted in the complete disappearance of endonuclease activity of the original cas9 protein, which only retained its ability to be guided into the genome by gRNA. This dcas9 protein has been codon optimized to make it more suitable for yeast.
  
 
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Latest revision as of 16:33, 13 October 2019


dCas9

dcas9 is a mutant of cas9 protein. The simultaneous mutation of RuvC1 and HNH nuclease activity regions in cas9 endonuclease resulted in the complete disappearance of endonuclease activity of the original cas9 protein, which only retained its ability to be guided into the genome by gRNA. This dcas9 protein has been codon optimized to make it more suitable for yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1075
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1156